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An optmized protocol for simultaneous extraction of DNA and RNA from soils BJM
Costa,Rodrigo; Gomes,Newton C.M.; Milling,Annett; Smalla,Kornelia.
In this work we report an optimized protocol for simultaneous extraction of DNA and RNA from soil matrices. Treatment of soil matrices with ethanol followed by bead-beating worked as a successful strategy to lyse the cells without considerable degradation of nucleic acids, resulting in DNA and RNA of good yield and integrity. The reverse transcribed RNA could be amplified with primers targeting a glutamine synthetase (glnA) gene fragment. From both DNA and cDNA, 16S rDNA fragments were amplified and analyzed by Denaturing Gradient Gel Electrophoresis (DGGE). The method was applied to soil and rhizosphere (strawberry and oilseed rape) samples. Two other protocols for the extraction of nucleic acids from soil were applied to the same set of samples in order...
Tipo: Info:eu-repo/semantics/article Palavras-chave: RNA extraction; Nucleic acids; Rhizosphere; Microbial communities.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200011
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Applications of nucleoside-based molecular probes for the in vivo assessment of tumour biochemistry using positron emission tomography (PET) BABT
Wiebe,Leonard I..
Positron emission tomography (PET) is a non-invasive nuclear imaging technique. In PET, radiolabelled molecules decay by positron emission. The gamma rays resulting from positron annihilation are detected in coincidence and mapped to produce three dimensional images of radiotracer distribution in the body. Molecular imaging with PET refers to the use of positron-emitting biomolecules that are highly specific substrates for target enzymes, transport proteins or receptor proteins. Molecular imaging with PET produces spatial and temporal maps of the target-related processes. Molecular imaging is an important analytical tool in diagnostic medical imaging, therapy monitoring and the development of new drugs. Molecular imaging has its roots in molecular biology....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Molecular biology; Molecular imaging; PET; Nucleosides; Nucleotides; Nucleic acids; Oligonucleotides; Antisense; Aptamer; Spiegelmer.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000300011
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Estudio de las concentraciones de los ácidos nucleicos (RNA, DNA)en músculo blanco e hígado de la lisa (Mugil brasiliensis)a lo largo de sus períodos de reposo y maduración gonadal OceanDocs
Duhalde, G.B.; Introzzi, A.E..
The concentration of nucleic acids (RNA and DNA)was reviewed, as well as the RNA/DNA coefficient, in white muscle and liver of Mugil brasiliensis adult females caught at the Mar Chiquita Coastal Laggon (37°30'S, 57°20'W)between September and March. Countrary to what observations in other species (Lepomis macrochirus and Clarias batrachus)have shown, i.e., a decrease in muscle RNA/DNA during gonadal maturation, Mugil brasiliensis would seem to maintain a proteic balance in equilibrium during the said period. No significant correlation was found between IGS and the RNA, DNA and RNA/DNA muscular variables.
Tipo: Journal Contribution Palavras-chave: Sexual maturity; Nucleic acids; DNA; Nucleic acids; Sexual maturity.
Ano: 1986 URL: http://hdl.handle.net/1834/2035
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Evolution des acides nucleiques au cours du developpement larvaire de la crevette rose Palaemon serratus (Pennant). ArchiMer
Campillo, Albert; Regnault, M.; Luquet, P..
The evolution of nucleic acids in larvae of P. serratus reared in the laboratory from hatching to metamorphosis has been studied. From the 1st larval stage to the post-larval stage, DNA and RNA concn decrease: from 185 to 95 uMoles-bases/g PSD for DNA and from 133.5 to 78.75 uMoles-bases for RNA. The concn decrease is not regular; it is emphasized between stages 4 and 5 on the one hand, and between stage 7 and the post-larva on the other hand. The amount of DNA and RNA per larva increases proportionally to the wet weight from stage 1 to the post-larva. As the weight is multiplied by 7.6, total DNA and total RNA are multiplied by 3.80 and 4.25 respectively. The RNA/DNA ratio has a mean value of 0.77; it increases slightly from stage 1 (0.72) to the...
Tipo: Text Palavras-chave: Calliphora; Crangon crangon; Palaemon serratus; Nucleic acids; Larval development; Invertebrate larvae; Biochemistry; Physiology.
Ano: 1975 URL: http://archimer.ifremer.fr/doc/1975/publication-2023.pdf
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Study of sodium arsenite induced biochemical changes on certain biomolecules of the freshwater catfish Clarias batrachus Neotropical Ichthyology
Kumar,Randhir; Banerjee,T. K..
Toxic impact of sublethal concentration (1 mg/L; 5% of 96h LC50 value) of sodium arsenite (NaAsO2) on certain biomolecules (proteins, nucleic acids, lipids, and glycogen) of five tissue components (muscles, liver, brain, skin, and gills) of the freshwater catfish Clarias batrachus was analysed. The important toxic manifestations include marked decrease in the concentration of proteins (21.72-45.42% in muscles; 3.42-53.94% in liver; 15.39-45.42% in brain; 15.40-4.00% in skin and 11.35-64.13% in gills), DNA (0.55-22.95% in muscles; 8.33-14.06% in liver; 5.30-18.40% in brain; 13.57-52.80% in skin; and 12.38-31.01% in gills), RNA (42.68-76.16% in muscles; 10.68-39.75% in liver; 5.66-29.05% in brain; 7.72-27.93% in skin and 21.47-44.38% in gills) and glycogen...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycogen; Lipids; Nucleic acids; Proteins; Toxicity.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252012000200021
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